PREPROCESSING AND REGISTRATION OF MINISCOPE-BASED CALCIUM IMAGING OF THE RODENT BRAIN

Microscopic imaging is central to the brain and cognition studies in animals and often requires advanced image processing. In vivo recordings on awake behaving animals require stabilization of the images as the tissue in the images undergoes non-rigid deformations due to animal movement, pulse beat and breathing of the animal. Here we propose an approach to compensation for the tissue motion in calcium imaging data acquired with miniaturized wearable microscopes (miniscopes) from live rodent brains. Our approach includes preprocessing of the images in which we compensate for non-uniform illumination, remove calcium transients and instrument-specific noise. For image registration we use the multiscale mutual information based non-rigid algorithm with B-spline transformation model. We present the preliminary results of such motion compensation approach applied to the real miniscope image stacks

26th Annual Computational Neuroscience Meeting (CNS*2017): Part 3 - Meeting Abstracts - Antwerp, Belgium. 15–20 July 2017

This work was produced as part of the activities of FAPESP Research,\ud Disseminations and Innovation Center for Neuromathematics (grant\ud 2013/07699-0, S. Paulo Research Foundation). NLK is supported by a\ud FAPESP postdoctoral fellowship (grant 2016/03855-5). ACR is partially\ud supported by a CNPq fellowship (grant 306251/2014-0)

Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy

This paper presents a nonivasive approach to study redox state of reduced cytochromes [Image: see text], [Image: see text] and [Image: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Image: see text], [Image: see text] and [Image: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Image: see text], [Image: see text] and [Image: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Image: see text], [Image: see text] and [Image: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Image: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data